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KMID : 0360220130540091429
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Journal of the Korean Ophthalmological Society
2013 Volume.54 No. 9 p.1429 ~ p.1434
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A Study of the Pathway of Nitric Oxide Production by Nitroglycerin in Trabecular Meshwork Cells
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Yoon Suk-Hyun
Kim Jae-Woo
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Abstract
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Purpose: To investigate the effects of nitroglycerin on the production of nitric oxide and its related pathway in cultured human trabecular meshwork cells (HTMC).
Methods: Primarily cultured HTMC were exposed to 10 nM nitroglycerin using 1% serum-containing media for 30 minutes. The production of nitric oxide was assessed with the Griess assay and expressions of eNOS mRNA was assessed with RT-PCR. Additionally, the cells were exposed to wortmanin and Akt1/2 kinase inhibitor to investigate the mechanism related to the production of nitric oxide.
Results: Nitroglycerin increased the production of nitric oxide (p < 0.05) accompanied with increased expression of eNOS mRNA. The increased production of nitric oxide and eNOS mRNA was inhibited by wortmanin and Akt1/2 kinase inhibitor.
Conclusions: Low-dose nitroglycerin increased the production of nitric oxide accompanied by increased eNOS activity. Nitroglycerin drives eNOS activation via the phosphatidylinositol 3-kinase/protein kinase B pathway.
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KEYWORD
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eNOS, Nitroglycerin, Nitric oxide, Trabecular meshwork cells
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